12:23 hour is come. Previously, Jesus had frequently responded to certain pressures merely by saying that His hour had not yet come (e.g., John 2:4; 7:6). Now, however, this was His final week, and He proceeded to stress that fact.
12:24 die. Before He could be glorified, however, Christ must die, be buried, then rise again. This is similar to a corn of wheat planted and seeming to die before springing up from the ground to produce life-giving bread.
12:25 shall lose it. The importance of this principle is indicated by the fact that Christ cites it probably more than any other of His teachings. See also Matthew 10:39; 16:25; Mark 8:35; Luke 9:24; 17:33. In slightly different form, it is also enunciated frequently by Paul (Romans 12:1-2; II Corinthians 5:14-15; 6:9-10; Galatians 2:20; Philippians 2:5-11; II Timothy 2:11-12).
12:26 where I am. And where is Jesus? “Where two or three are gathered together in my name, there am I in the midst of them” (Matthew 18:20). Such an assembly would usually (but not necessarily) be in an organized local church.
12:26 my servant. In this verse, the two occurrences of “serve” should be read in the sense of “minister to.” The word “servant, however, means “bondslave.”

